iwp l6 abmole cat (Tocris)
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iwp l6 abmole cat
Iwp L6 Abmole Cat, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iwp+l6/IWP+L6/pm36313798-187-209-207
Average 93 stars, based on 6 article reviews
Iwp L6 Abmole Cat, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iwp+l6/IWP+L6/pm36313798-187-209-207
Average 93 stars, based on 6 article reviews
iwp l6 abmole cat - by Bioz Stars,
2026-09
93/100 stars
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Immunostaining:Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells. Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 mM of XAV939 ( Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 μM of XAV939 (Tocris) and 0.25 μM IWP-L6 (AbMole).. From differentiation d4 on, the cells were maintained in mBEL medium.From differentiation d4 on, the cells were maintained in mBEL medium. Article Title: Large-scale genome editing based on high-capacity adenovectors and CRISPR-Cas9 nucleases rescues full-length dystrophin synthesis in DMD muscle cells Article Snippet: At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).. Two days later, the medium was replenished with mBEL medium supplemented with 5 μM XAV 939 (Tocris; Cat. No.: 3748/10) and 0.25 μM IWP-L6 (AbMole; Cat. No.: M2781).. Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056).Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056). Article Title: STRAIGHT-IN: A platform for high-throughput targeting of large DNA payloads into human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 µM of XAV939 ( Article Title: Deletion of the Dishevelled family of genes disrupts anterior-posterior axis specification and selectively prevents mesoderm differentiation. Article Snippet: The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.. The following drug treatments were added at the time of plating and removed after 48 h: 100 ng/ml Activin A (R&D Systems), 50 ng/ml BMP4 (R&D Systems), 3uM CHIR-99021 (Tocris), 100 ng/ml Wnt3a (R&D Systems), 10 μM SB-431542 (EMD Millipore), 200 nM LDN-193189 (Stemgent), Inhibition:Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells. Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 mM of XAV939 ( Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 μM of XAV939 (Tocris) and 0.25 μM IWP-L6 (AbMole).. From differentiation d4 on, the cells were maintained in mBEL medium.From differentiation d4 on, the cells were maintained in mBEL medium. Article Title: Large-scale genome editing based on high-capacity adenovectors and CRISPR-Cas9 nucleases rescues full-length dystrophin synthesis in DMD muscle cells Article Snippet: At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).. Two days later, the medium was replenished with mBEL medium supplemented with 5 μM XAV 939 (Tocris; Cat. No.: 3748/10) and 0.25 μM IWP-L6 (AbMole; Cat. No.: M2781).. Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056).Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056). Article Title: STRAIGHT-IN: A platform for high-throughput targeting of large DNA payloads into human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 µM of XAV939 ( Article Title: Deletion of the Dishevelled family of genes disrupts anterior-posterior axis specification and selectively prevents mesoderm differentiation. Article Snippet: The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.. The following drug treatments were added at the time of plating and removed after 48 h: 100 ng/ml Activin A (R&D Systems), 50 ng/ml BMP4 (R&D Systems), 3uM CHIR-99021 (Tocris), 100 ng/ml Wnt3a (R&D Systems), 10 μM SB-431542 (EMD Millipore), 200 nM LDN-193189 (Stemgent), Quantitative RT-PCR:Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells. Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 mM of XAV939 ( Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 μM of XAV939 (Tocris) and 0.25 μM IWP-L6 (AbMole).. From differentiation d4 on, the cells were maintained in mBEL medium.From differentiation d4 on, the cells were maintained in mBEL medium. Article Title: Large-scale genome editing based on high-capacity adenovectors and CRISPR-Cas9 nucleases rescues full-length dystrophin synthesis in DMD muscle cells Article Snippet: At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).. Two days later, the medium was replenished with mBEL medium supplemented with 5 μM XAV 939 (Tocris; Cat. No.: 3748/10) and 0.25 μM IWP-L6 (AbMole; Cat. No.: M2781).. Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056).Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056). Article Title: STRAIGHT-IN: A platform for high-throughput targeting of large DNA payloads into human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 µM of XAV939 ( Article Title: Deletion of the Dishevelled family of genes disrupts anterior-posterior axis specification and selectively prevents mesoderm differentiation. Article Snippet: The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.. The following drug treatments were added at the time of plating and removed after 48 h: 100 ng/ml Activin A (R&D Systems), 50 ng/ml BMP4 (R&D Systems), 3uM CHIR-99021 (Tocris), 100 ng/ml Wnt3a (R&D Systems), 10 μM SB-431542 (EMD Millipore), 200 nM LDN-193189 (Stemgent), Expressing:Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells. Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 mM of XAV939 ( Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 μM of XAV939 (Tocris) and 0.25 μM IWP-L6 (AbMole).. From differentiation d4 on, the cells were maintained in mBEL medium.From differentiation d4 on, the cells were maintained in mBEL medium. Article Title: Large-scale genome editing based on high-capacity adenovectors and CRISPR-Cas9 nucleases rescues full-length dystrophin synthesis in DMD muscle cells Article Snippet: At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).. Two days later, the medium was replenished with mBEL medium supplemented with 5 μM XAV 939 (Tocris; Cat. No.: 3748/10) and 0.25 μM IWP-L6 (AbMole; Cat. No.: M2781).. Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056).Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056). Article Title: STRAIGHT-IN: A platform for high-throughput targeting of large DNA payloads into human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 µM of XAV939 ( Article Title: Deletion of the Dishevelled family of genes disrupts anterior-posterior axis specification and selectively prevents mesoderm differentiation. Article Snippet: The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.. The following drug treatments were added at the time of plating and removed after 48 h: 100 ng/ml Activin A (R&D Systems), 50 ng/ml BMP4 (R&D Systems), 3uM CHIR-99021 (Tocris), 100 ng/ml Wnt3a (R&D Systems), 10 μM SB-431542 (EMD Millipore), 200 nM LDN-193189 (Stemgent), Activation Assay:Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells. Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 mM of XAV939 ( Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 μM of XAV939 (Tocris) and 0.25 μM IWP-L6 (AbMole).. From differentiation d4 on, the cells were maintained in mBEL medium.From differentiation d4 on, the cells were maintained in mBEL medium. Article Title: Large-scale genome editing based on high-capacity adenovectors and CRISPR-Cas9 nucleases rescues full-length dystrophin synthesis in DMD muscle cells Article Snippet: At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).. Two days later, the medium was replenished with mBEL medium supplemented with 5 μM XAV 939 (Tocris; Cat. No.: 3748/10) and 0.25 μM IWP-L6 (AbMole; Cat. No.: M2781).. Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056).Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056). Article Title: STRAIGHT-IN: A platform for high-throughput targeting of large DNA payloads into human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 µM of XAV939 ( Article Title: Deletion of the Dishevelled family of genes disrupts anterior-posterior axis specification and selectively prevents mesoderm differentiation. Article Snippet: The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.. The following drug treatments were added at the time of plating and removed after 48 h: 100 ng/ml Activin A (R&D Systems), 50 ng/ml BMP4 (R&D Systems), 3uM CHIR-99021 (Tocris), 100 ng/ml Wnt3a (R&D Systems), 10 μM SB-431542 (EMD Millipore), 200 nM LDN-193189 (Stemgent), Marker:Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells. Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 mM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 mM of XAV939 ( Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 μM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 μM of XAV939 (Tocris) and 0.25 μM IWP-L6 (AbMole).. From differentiation d4 on, the cells were maintained in mBEL medium.From differentiation d4 on, the cells were maintained in mBEL medium. Article Title: Large-scale genome editing based on high-capacity adenovectors and CRISPR-Cas9 nucleases rescues full-length dystrophin synthesis in DMD muscle cells Article Snippet: At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).At 24 h after seeding, the medium was replaced with modified LI-BPEL (mBEL) medium ( ) supplemented with 5 μM CHIR 99021 (Axon Medchem; Cat. No.: Axon1386).. Two days later, the medium was replenished with mBEL medium supplemented with 5 μM XAV 939 (Tocris; Cat. No.: 3748/10) and 0.25 μM IWP-L6 (AbMole; Cat. No.: M2781).. Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056).Finally, after an additional 2-day period the medium was replenished with mBEL medium supplemented with Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (1:1000) (ThermoFisher; Cat. No.: 51500056). Article Title: STRAIGHT-IN: A platform for high-throughput targeting of large DNA payloads into human pluripotent stem cells Article Snippet: On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).On d0, the cells were refreshed with mBEL medium containing 5 µM CHIR99021 (Axon Medchem).. On differentiation d2, the cells were refreshed with mBEL medium containing 5 µM of XAV939 ( Article Title: Deletion of the Dishevelled family of genes disrupts anterior-posterior axis specification and selectively prevents mesoderm differentiation. Article Snippet: The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.The cells were cultured in IMDM supplemented with 20% FBS, 1% nonessential amino acids, 1% GlutaMAX, 1% penicillin/streptomycin, and β-mercaptoethanol.. The following drug treatments were added at the time of plating and removed after 48 h: 100 ng/ml Activin A (R&D Systems), 50 ng/ml BMP4 (R&D Systems), 3uM CHIR-99021 (Tocris), 100 ng/ml Wnt3a (R&D Systems), 10 μM SB-431542 (EMD Millipore), 200 nM LDN-193189 (Stemgent), |
